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T cell tyrosine phosphorylation response to transient redox stress

Secchi, Christian and Carta, Marissa and Crescio, Claudia and Spano, Alessandra and Arras, Marcella and Caocci, Giovanni and Galimi, Francesco and La Nasa, Giorgio and Pippia, Proto Gavino and Turrini, Francesco Michelangelo and Pantaleo, Antonella (2015) T cell tyrosine phosphorylation response to transient redox stress. Cellular signalling, Vol. 27 (4), p. 777-788. ISSN 0898-6568. eISSN 1873-3913. Article.

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DOI: 10.1016/j.cellsig.2014.12.014

Abstract

Reactive Oxygen Species (ROS) are crucial to multiple biological processes involved in the pathophysiology of inflammation, and are also involved in redox signaling responses. Although previous reports have described an association between oxidative events and the modulation of innate immunity, a role for redox signaling in T cell mediated adaptive immunity has not been described yet. This work aims at assessing if T cells can sense redox stress through protein sulfhydryl oxidation and respond with tyrosine phosphorylation changes. Our data show that Jurkat T cells respond to –SH group oxidation with specific tyrosine phosphorylation events. The release of T cell cytokines TNF, IFNγ and IL2 as well as the expression of a number of receptors are affected by those changes. Additionally, experiments with spleen tyrosine kinase (Syk) inhibitors showed a major involvement of Syk in these responses. The experiments described herein show a link between cysteine oxidation and tyrosine phosphorylation changes in T cells, as well as a novel mechanism by which Syk inhibitors exert their anti-inflammatory activity through the inhibition of a response initiated by ROS.

Item Type:Article
ID Code:10640
Status:Published
Refereed:Yes
Uncontrolled Keywords:Cysteine oxidation, tyrosine phosphorylation, inflammation, T cells, kinases & phosphatases, post-translational protein modifications, Syk inhibitors
Subjects:Area 05 - Scienze biologiche > BIO/17 Istologia
Area 05 - Scienze biologiche > BIO/06 Anatomia comparata e citologia
Area 05 - Scienze biologiche > BIO/09 Fisiologia
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
002 Altri enti e centri di ricerca del Nord Sardegna > Istituto nazionale biostrutture e biosistemi, Unità di ricerca, Sassari
Publisher:Elsevier
ISSN:0898-6568
eISSN:1873-3913
Deposited On:19 Jan 2015 11:10

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