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An LC—MS—MS method for quantitation of four new phenethylamines (BOX series) in plasma: in vivo application

Boatto, Gianpiero and Pirisi, Maria Antonietta and Burrai, Lucia and Baralla, Elena and Demontis, Maria Piera and Varoni, Maria Vittoria and Nieddu, Maria (2014) An LC—MS—MS method for quantitation of four new phenethylamines (BOX series) in plasma: in vivo application. Forensic Toxicology, Vol. 32 (1), p. 75-81. ISSN 1860-8965. eISSN 1860-8973. Article.

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DOI: 10.1007/s11419-013-0204-7

Abstract

The appearance of new “designer drugs” in the illicit market poses a serious health risk because they have unknown safety profiles, have a high potential for abuse, high potency, and can lead to devastating health consequences. For this reason, it is desirable to develop validated and reliable analytical screening tests that allow detection of amphetamines and related designer drugs in biological samples. We report a method for separation and quantitation of four new phenethylamines, 4-bromo-2,5-beta-trimethoxyphenethylamine (BOB), 4-methyl-2,5-beta-trimethoxyphenethylamine (BOD), 3,4-methylenedioxy-beta-methoxyphenethylamine (BOH), and 4-methyl-2,5-dimethoxy-beta-hydroxyphenethylamine (BOHD), in plasma. Quantitation was achieved via liquid chromatography—tandem mass spectrometry (LC—MS—MS) in the multiple reaction monitoring mode, using 2,3-dimethoxyphenethylamine-d 3 as internal standard. The method was validated according to international guidelines. The parameters determined were selectivity, sensitivity, matrix effect, linearity, precision, recovery, and stability. All parameters were satisfactory. To remove matrix interference, solid-phase extraction was introduced in the method as clean-up step. The same method was applied in a pharmacokinetic study to monitor the target compounds in rat plasma after a single oral administration. The developed and validated LC—MS—MS method is the first available for quantitation of BOB, BOH, BOD, and BOHD in a biological matrix. This method is recommended for use in forensic and clinical toxicology, because of its sensitivity, selectivity, and simplicity. An important extension of this method could involve its application to other complex matrices.

Item Type:Article
ID Code:10554
Status:Published
Refereed:Yes
Uncontrolled Keywords:BOX series, phenethylamines, LC—MS—MS, plasma, pharmacokinetic profile
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/07 Farmacologia e tossicologia veterinaria
Area 03 - Scienze chimiche > CHIM/08 Chimica farmaceutica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Chimica e Farmacia
Publisher:Springer Japan
ISSN:1860-8965
eISSN:1860-8973
Deposited On:22 Dec 2014 17:56

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