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Evaluation of non-covalent interactions between serum albumin and green tea catechins by affinity capillary electrophoresis

Zinellu, Angelo and Sotgia, Salvatore and Scanu, Bastianina and Pisanu, Elisabetta and Giordo, Roberta and Cossu, Annalisa and Posadino, Anna Maria and Carru, Ciriaco and Pintus, Gianfranco (2014) Evaluation of non-covalent interactions between serum albumin and green tea catechins by affinity capillary electrophoresis. Journal of Chromatography A, Vol. 1367 , p. 167-171. ISSN 0021-9673. Article.

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DOI: 10.1016/j.chroma.2014.09.053

Abstract

The natural antioxidant-associated biological responses appear contradictory since biologically active dosages registered in vitro experiments are considerably higher if compared to concentrations found in vivo. The recent research indicates that natural antioxidants, including the major catechins of green tea epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) form non-covalent complexes with albumin, a crucial aspect that may modulate their plasma concentration, tissue delivery and biological activity. Affinity capillary electrophoresis (ACE) was used to characterize the binding of the four catechins to human serum albumin (HSA) and bovine serum albumin (BSA) at near-physiological conditions: 10 mmol/L phosphate buffer, HEPES 50 mmol/L (pH 7.5), temperature 37 °C. The studied flavonoids displayed affinities toward the albumin with binding constants in the range 103−105 M−1, with a greater affinity of catechins toward HSA than BSA (between 3 and 3.5 fold higher). We also confirmed that catechins having a galloyl moiety (ECG and EGCG) have a higher binding affinity toward albumin than the catechins lacking the galloyl moiety (EC and EGC), and that for both albumins the order of affinity is EC < EGC < ECG < EGCG. We believe that our work can provide useful information for better understanding the intercurrent relationships between cathechins bioavailability and their elicited biological effects.

Item Type:Article
ID Code:10515
Status:Published
Refereed:Yes
Uncontrolled Keywords:Affinity capillary electrophoresis, green tea, catechins, albumin, binding constant
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Area 05 - Scienze biologiche > BIO/12 Biochimica clinica e biologia molecolare clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Elsevier Science
ISSN:0021-9673
Deposited On:03 Dec 2014 13:32

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