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Ricerca e caratterizzazione di Vibrio parahaemolyticus in molluschi bibalvi vivi della Regione Sardegna

Marceddu, Marta (2015) Ricerca e caratterizzazione di Vibrio parahaemolyticus in molluschi bibalvi vivi della Regione Sardegna. Doctoral Thesis.

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Abstract

During 2013, 1079 mollusk samples collected from Sardinia ASLs (754 for Monitoring and 325 for market surveillance) were analyzed by IZS of Sassari. Samples were oysters (Crassostrea gigas, Ostrea edulis), clams (Tapes philippinarum and decussatus) and mussels (Mytilus galloprovincialis and edulis). Samples were analyzed for V. parahaemolyticus by the ISO/TS 21872-1:2007. Strains were tested by PCR for the presence of V. parahaemolyticus species-specific genes (toxR) and to detect the virulence genes (tdh and trh). Group specific (GS)-PCR method, based on the sequence variation in ToxRS gene, was also performed to detect the pandemic clone in correlation with tdh gene. V. parahemolyticus prevalence was 3.5% (38/1079). between positive samples, 35 (92.1%) were for Monitoring and 3 (7.9%) for official controls. tdh gene was identified In 5 (13%) samples, but there was no correlation with toxRS genes. Furthermore, trh gene was detected in 10 (26.3%) samples. V. parahaemolyticus is responsible of emerging public health problems. Reg. CE 2073/2005 does not fix specific criteria, however, recommend the application of good hygienic practices. V.parahaemolyticus prevalence is low. However, such results confirm that it is necessary acquire more knowledge about V. parahaemolyticus in Sardinia marine seawater. It would be appropriate to include this microorganism in the surveillance systems for gastrointestinal diseases and in monitoring programs in mollusk collection areas.

Item Type:Doctoral Thesis
ID Code:10450
Contributors:Mazzette, Rina and Virgilio, Sebastiano
Publisher:Università degli studi di Sassari
Uncontrolled Keywords:Vibrio parahaemolyticus, molluschi bivalvi, prevalenza, caratterizzazione
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/04 Ispezione degli alimenti di origine animale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Cicli, scuole e corsi:Ciclo 27 > Scienze Veterinarie > Produzione, qualità e sicurezza degli alimenti di origine animale
Deposited On:18 Mar 2015 10:08

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