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Isolamento e valutazione degli effetti di microrganismi entomopatogeni nei confronti di insetti di interesse agrario, forestale e medico-veterinario

Virdis, Barbara (2015) Isolamento e valutazione degli effetti di microrganismi entomopatogeni nei confronti di insetti di interesse agrario, forestale e medico-veterinario. Doctoral Thesis.

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Abstract

The insecticidal potential of various entomopathogenic bacteria was evaluated by ingestion and/or injection bioassays against noxious insects including Musca domestica, Ceratitis capitata, Lymantria dispar, and Malacosoma neustria. Bacteria involved in the study included newly isolated soil occurring strains, reference strains (i.e. Bacillus thuringiensis, Brevibacillus laterosporus, Photorhabdus luminescens and Xenorhabdus nematophila), and others isolated form Galleria mellonella larvae infected with an original collection of entomopathogenic nematods. The new bacterial isolates were identified by phylogenetic analysis involving 16S rRNA gene sequencing.
As a result of bioassays, significant differences in the insecticidal potential emerged for diverse bacterial strains and different insect targets. B. thuringiensis, P. luminescines and X. nematophila were generally highly toxic against lepidopteran larvae, while different B. laterosporus strains showed variable degrees of toxicity against the house fly.
Among the others, significant was the pathogenic potential of Bacillus firmus, Paenibacillus tundrae and Bacillus psychrodurans, and of new species belonging to the genera Serratia, Pseudomonas, Stenotrophomonas and Alcaligenes.
These results are discussed in comparison with previous knowledge in the field, highlighting the prospect of a possible use of new bacterial strains for further studies focusing on the development of new actives for the microbial pest management.

Item Type:Doctoral Thesis
ID Code:10439
Contributors:Floris, Ignazio
Publisher:Università degli studi di Sassari
Uncontrolled Keywords:Entomopathogens, biological control, housefly, bioassays, microbes
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/11 Entomologia generale e applicata
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
Cicli, scuole e corsi:Ciclo 26 > Scienze e Biotecnologie dei sistemi agrari e forestali e delle produzioni alimentari > Monitoraggio e controllo degli ecosistemi forestali in ambiente mediterraneo
Deposited On:18 Mar 2015 12:49

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