titoli, abstracts, parole chiave >>>
Listeria monocytogenes persistence in ready-to-eat sausages and in processing plants

Mureddu, Anna and Mazza, Roberta and Fois, Federica and Meloni, Domenico and Bacciu, Roberto and Piras, Francesca and Mazzette, Rina (2014) Listeria monocytogenes persistence in ready-to-eat sausages and in processing plants. Italian Journal of Food Safety = Rivista dell'Associazione Italiana dei Veterinari Igienisti, Vol. 3 (1), p. 12-15. ISSN 2239-7132. Article.

Full text not available from this repository.

DOI: 10.4081/ijfs.2014.1697

Abstract

Listeria monocytogenes is of major concern in the fermented meat products and is able to persist in their processing environments. The aim of the present work was to evaluate the virulence profile and the persistence capacity of L. monocytogenes strains isolated in Sardinian fermented sausages processing plants. Food (ground meat, sausages at the end of acidification and ripening stage) and environmental samples (a total of n. 385), collected from 4 meat processing plants located in Sardinia (Italy), were examined to detect L. monocytogenes presence. All the L. monocytogenes isolates were identified by polymerase chain reaction (PCR) method. A subset of strains was also characterised by multiplex PCR-based serogrouping, using the lmo0737, lmo1118, ORF2819 and ORF2110 genes. Three different multiplex PCRs were used to obtain the virulence profiles by the rrn, hlyA, actA, prfA, inlA, inlB, iap, plcA, plcB and mpl marker genes. Furthermore, in vitro biofilm forming ability and resistance to disinfectants were carried out on microtiter plate. The overall prevalence was 31.5% in food, and 68.5% in environmental samples. The prevalent serotype resulted 1/2c (43%), followed by 1/2a (40%), 4b (8.6%), and 1/2b (8.6%). The amplification products of the virulence genes were found in all the isolates with the following prevalence: 77.1% hlyA; 100% rrn; 100% prfA; 97.1% iap; 65.7% inlB; 88.6% inlA; 100% plcA; 100% plcB and 74.3% mpl. As for biofilm forming ability, 37.1% of the strains were positive and resulted weak producer, but all the isolates were sensible to disinfectants showing a reduction of L. monocytogenes growth after each incubation time. More appropriate technologies and application of measures of hygienic control should be implemented to prevent the L. monocytogenes growth and crosscontamination in salsiccia sarda processing plants.

Item Type:Article
ID Code:10367
Status:Published
Refereed:Yes
Uncontrolled Keywords:Listeria monocytogenes, salsiccia sarda, biofilm, disinfectant resistance
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/04 Ispezione degli alimenti di origine animale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:AIVI
ISSN:2239-7132
Deposited On:24 Nov 2014 09:46

I documenti depositati in UnissResearch sono protetti dalle leggi che regolano il diritto d'autore

Repository Staff Only: item control page