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Endocrine disruptors differently influence estrogen receptor β and androgen receptor in male and female rat VSMC

Pellegrini, Marco and Bulzomi, Pamela and Lecis, Marco and Leone, Stefano and Campesi, Ilaria and Franconi, Flavia and Marino, Maria (2014) Endocrine disruptors differently influence estrogen receptor β and androgen receptor in male and female rat VSMC. Journal of cellular physiology, Vol. 229 (8), p. 1061-1068. ISSN 0021-9541. eISSN 1097-4652. Article.

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DOI: 10.1002/jcp.24530

Abstract

Sex steroid hormones differently control the major physiological processes in male and female organisms. In particular, their effects on vascular smooth muscle cells (VSMCs) migration are at the root of sex/gender-related differences reported in the cardiovascular system. Several exogenous substances, defined endocrine disruptor chemicals (EDCs), could interfere with these androgen and estrogen effects; however, the sex/gender-related susceptibility of VSMC motility to EDCs is completely unknown. Here, the effect of naturally occurring (naringenin, Nar) and synthetic (bisphenol A, BPA) EDCs on male and female VSMC motility has been evaluated. 17β-estradiol (E2, 0.1 nM–1 µM) induced a dose-dependent inhibition of motility in female-derived VSMC. In contrast, neither dihydrotestosterone (DHT, 0.01–100 nM) nor the common precursor of sex steroid hormones, testosterone (Tes, 0.01–100 nM) modified male-derived VSMC motility. Estrogen receptor (ER) β subtype-dependent activation of p38 was necessary for the E2 effect on cell motility. High BPA concentration prevented E2 effects in female-derived cells being without any effect in male-derived cells. Nar mimicked E2 effects on female-derived cells even in the presence of E2 or BPA. Intriguingly, Nar also inhibited the male-derived VSMC mobility. This latter effect was prevented by ERβ inhibitor, but not by the androgen receptor (AR) inhibitor. As a whole, ERβ-dependent signals in VSMC results more susceptible to the impact of EDCs than AR signals suggesting a possible high and overall susceptibility of female to EDCs. However, several male-derived cells, including VSMC, express ERβ, which could also serve as target of EDC disruption in male organisms.

Item Type:Article
ID Code:10336
Status:Published
Refereed:Yes
Uncontrolled Keywords:Sex steroid hormones, vascular smooth muscle cells (VSMCs), cardiovascular system
Subjects:Area 05 - Scienze biologiche > BIO/14 Farmacologia
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Chirurgiche, Microchirurgiche e Mediche
002 Altri enti e centri di ricerca del Nord Sardegna > National laboratory of the national institute of biostructures and biosystems, Osilo
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Wiley
ISSN:0021-9541
eISSN:1097-4652
Deposited On:18 Nov 2014 10:31

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