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A Culture-independent PCR-based method for the detection of Lachancea thermotolerans in wine

Zara, Giacomo and Ciani, Maurizio and Domizio, Paola and Zara, Severino and Budroni, Marilena and Carboni, Adriana and Mannazzu, Ilaria Maria (2014) A Culture-independent PCR-based method for the detection of Lachancea thermotolerans in wine. Annals of Microbiology, Vol. 64 (1), p. 403-406. ISSN 1590-4261. eISSN 1869-2044. Article.

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DOI: 10.1007/s13213-013-0647-4


When inoculated in association with Saccharomyces cerevisiae, the yeast Lachancea thermotolerans determines a reduction of volatile acidity and an increase in the production of glycerol, 2-phenylethanol, and polysaccharides. Moreover, L. thermotolerans is a natural L-lactic acid producer, thus it contributes to wine acidification and microbiological stabilization. In view of its utilization in winemaking, a culture-independent PCR-based method was developed for the detection of L. thermotolerans during wine fermentations. This method, which utilizes species-specific PCR primer pairs that anneal to intron 2 of the mitochondrial COX1 gene, is rapid and reliable, and detects L. thermotolerans in wine at 104 cells/ml and with a S. cerevisiae/L. termotholerans ratio of 1,000/1.

Item Type:Article
ID Code:10306
Uncontrolled Keywords:Lachancea thermotolerans, wine, mixed starters, COX1
Subjects:Area 07 - Scienze agrarie e veterinarie > AGR/16 Microbiologia agraria
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Agraria
Publisher:Springer Berlin Heidelberg
Deposited On:14 Nov 2014 13:46

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