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Polymorphism of cagA and vacA genes in Helicobacter pylori isolated from gastroduodenal diseas patients in Central Vietnam

Phan, Trung Nam (2015) Polymorphism of cagA and vacA genes in Helicobacter pylori isolated from gastroduodenal diseas patients in Central Vietnam. Doctoral Thesis.

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Abstract

H. pylori plays a significant role in the pathogenesis of upper alimentary tract diseases. Vietnam is a developing country with high prevalence of H. pylori infection and an intermediate risk of gastric cancer. The aim of this thesis was to determine the antibiogram and the molecular basis of the resistance pattern; to investigate cagA and vacA genotypes, and to determine a clinical relevance of cagA and vacA polymorphisms of H. pylori strains isolated from dyspeptic patients in Central Vietnam.
We examined dyspeptic patients who were referred to Hue University Hospital between July 2012 and January 2014. A total of 92 strains antibiogram was performed by E-test and 96 extracted DNA samples from 96 strains were available for analysis. Point mutations related to clarithromycin and levofloxacin on 23S rRNA and gyrA genes were detected by sequencing. vacA and cagA genotyping were carried out using PCR and sequencing, then their clinical relevance was further analyzed.
We highlight a very high resistance rate in Central Vietnam with 56.5% of multiple resistance and higher prevalence of secondary resistance versus primary resistance. Data on determinants of resistance to clarithomycin and levofloxacin are new information in Vietnam. cagA positive status, East Asian cagA type and vacA s1i1m1 type are the most predominant in gastric ulcer and gastric cancer. The prevalence of cagA negative status and Western cagA type is higher than that reported in previous studies in Vietnam.

Item Type:Doctoral Thesis
ID Code:10297
Contributors:Paglietti, Bianca and Rubino, Salvatore
Publisher:Università degli studi di Sassari
Uncontrolled Keywords:Helicobacter pylori, cagA, vacA, antibiotic resistance, gyrA, 23S rRNA
Subjects:Area 06 - Scienze mediche > MED/07 Microbiologia e microbiologia clinica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Cicli, scuole e corsi:Ciclo 27 > Biomolecular and Biotechnological Sciences > Microbiologia e Immunologia
Deposited On:18 Mar 2015 11:43

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