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Derangement of the T-cell repertoire in patients with B-cell non-Hodgkin's lymphoma

Fozza, Claudio and Corda, Giovanna and Virdis, Patrizia and Contini, Salvatore and Barraqueddu, Francesca and Galleu, Antonio and Isoni, Antonella and Cossu, Antonella and Dore, Fausto and Careddu, Maria Giovanna and Bonfigli, Silvana and Giannico, Barbara Domenica and Longinotti, Maurizio Roberto (2015) Derangement of the T-cell repertoire in patients with B-cell non-Hodgkin's lymphoma. European Journal of Haematology, Vol. 94 (4), p. 298-309. eISSN 1600-0609. Article.

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DOI: 10.1111/ejh.12417

Abstract

Although a number of studies suggest that different immune pathways may play a role in the pathogenesis of non-Hodgkin's lymphomas (NHL), the shape of the T-cell compartment has been only superficially explored in these patients. In our study, we analyzed the peripheral T-cell receptor (TCR) repertoire and the distribution of different T-cell subsets – including regulatory T cells (Treg) – in 30 patients with NHL, by combining flow cytometry and spectratyping. We first demonstrated by flow cytometry an increased frequency of expanded T-cell subpopulations expressing the same TCR beta variable (BV) subfamilies in CD8+ cells from NHL patients when compared with healthy controls, beside a higher frequency of Treg. Moreover, NHL patients were characterized by a higher percentage of BVs showing a skewed CDR3 profile both in CD4+ and CD8+ cells when analyzed by spectratyping. Our data suggest that the T-cell branch of the immune system of patients with B-cell NHL is deeply deranged, as witnessed by the increased degree of activation and skewing of their TCR repertoire along with the higher frequency of Treg.

Item Type:Article
ID Code:10231
Status:Published
Refereed:Yes
Uncontrolled Keywords:non-Hodgkin's lymphoma, T-cell receptor repertoire, regulatory T cells, flow cytometry, spectratyping, third complementarity determining region
Subjects:Area 06 - Scienze mediche > MED/15 Malattie del sangue
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Clinica e Sperimentale
Publisher:Blackwell / Wiley
eISSN:1600-0609
Deposited On:29 Oct 2014 09:23

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