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Presence and molecular characterization of the major serovars of Listeria monocytogenes in ten Sardinian fermented sausage processing plants

Meloni, Domenico and Consolati, Simonetta Gianna and Mazza, Roberta and Mureddu, Anna and Fois, Federica and Piras, Francesca and Mazzette, Rina (2014) Presence and molecular characterization of the major serovars of Listeria monocytogenes in ten Sardinian fermented sausage processing plants. Meat Science, Vol. 97 (4), p. 443-450. ISSN 0309-1740. eISSN 1873-4138. Article.

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DOI: 10.1016/j.meatsci.2014.02.012

Abstract

The aim of the present study was to investigate the occurrence of Listeria monocytogenes in ten Sardinian fermented sausage processing plants. A total of 230 samples were collected and 40 L. monocytogenes isolates were obtained and subjected to serotyping and investigated for the presence of ten virulence-associated genes using multiplex PCR assays. The isolates were further subjected to PFGE and investigated for their adhesion abilities in polystyrene microtiter plates. L. monocytogenes was found in 6% of food contact surfaces, in sausages at the end of acidification (3%) and ripening (8%). Serotyping revealed the presence of four serovars: 1/2c (37.5%), 1/2b (27.5%), 4b (22.5%) and 1/2a (12.5%). All virulence-associated genes were detected in 67.5% of the isolates. Isolates from processing environment, semi-processed and finished products showed high pulsotype diversity and the majority of isolates presented weak adhesion capability. The detection of the pathogen in fermented sausages confirms the ability of L. monocytogenes to overcome the hurdles of the manufacturing process.

Item Type:Article
ID Code:10221
Status:Published
Refereed:Yes
Uncontrolled Keywords:Adhesion ability, fermented sausage, Listeria monocytogenes, pathogenic profile, pulsotype, serovar
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/04 Ispezione degli alimenti di origine animale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Elsevier
ISSN:0309-1740
eISSN:1873-4138
Deposited On:24 Oct 2014 12:08

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