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Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model

Manca, Maria Elena and Manunta, Maria Lucia Gabriella M. and Spezzigu, Antonio and Torres Rovira, Laura and Gonzalez Bulnes, Antonio and Pasciu, Valeria and Piu, Peter and Leoni, Giovanni Giuseppe and Succu, Sara and Chesneau, Didier and Naitana, Salvatore and Berlinguer, Fiammetta (2014) Melatonin deprival modifies follicular and corpus luteal growth dynamics in a sheep model. Reproduction, Vol. 147 (6), p. 885-895. ISSN 1470-1626. eISSN 1741-7899. Article.

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DOI: 10.1530/REP-13-0405

Abstract

This study assessed the effect of melatonin deprival on ovarian status and function in sheep. Experimental procedures were carried out within two consecutive breeding seasons. Animals were divided into two groups: pinealectomised (n=6) and sham-operated (n=6). The completeness of the pineal gland removal was confirmed by the plasma concentration of melatonin. Ovarian status was monitored by ovarian ultrasonography for 1 year to study reproductive seasonality. Follicular and corpus luteal growth dynamics were assessed during an induced oestrous cycle. As the effects of melatonin on the ovary may also be mediated by its antioxidant properties, plasma Trolox equivalent antioxidant capacity (TEAC) was determined monthly for 1 year. Pinealectomy significantly extended the breeding season (310±24.7 vs 217.5±24.7 days in controls; P<0.05). Both pinealectomised and sham-operated ewes showed a well-defined wave-like pattern of follicle dynamics; however, melatonin deficiency caused fewer waves during the oestrous cycle (4.3±0.2 vs 5.2±0.2; P<0.05), because waves were 1 day longer when compared with the controls (7.2±0.3 vs 6.1±0.3; P<0.05). The mean area of the corpora lutea (105.4±5.9 vs 65.4±5.9 mm2; P<0.05) and plasma progesterone levels (7.1±0.7 vs 4.9±0.6 ng/ml; P<0.05) were significantly higher in sham-operated ewes compared with pinealectomised ewes. In addition, TEAC values were significantly lower in pinealectomised ewes compared with control ones. These data suggest that melatonin, besides exerting its well-known role in the synchronisation of seasonal reproductive fluctuations, influences the growth pattern of the follicles and the steroidogenic capacity of the corpus luteum.

Item Type:Article
ID Code:10208
Status:Published
Refereed:Yes
Uncontrolled Keywords:Melatonin, pinealectomised, sham-operated, plasma progesterone, corpora lutea
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/09 Clinica chirurgica veterinaria
Area 07 - Scienze agrarie e veterinarie > VET/02 Fisiologia veterinaria
Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Society for Reproduction and Fertility
ISSN:1470-1626
eISSN:1741-7899
Deposited On:23 Oct 2014 10:03

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