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Stem cell senescence: effects of REAC technology on telomerase-independent and telomerase-dependent pathways

Rinaldi, Salvatore and Maioli, Margherita and Pigliaru, Gianfranco and Castagna, Alessandro and Santaniello, Sara and Basoli, V. and Fontani, V. and Ventura, Carlo (2014) Stem cell senescence: effects of REAC technology on telomerase-independent and telomerase-dependent pathways. Scientific reports, Vol. 4 (6373). eISSN 2045-2322. Article.

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DOI: 10.1038/srep06373

Abstract

Decline in the gene expression of senescence repressor Bmi1, and telomerase, together with telomere shortening, underlay senescence of stem cells cultured for multiple passages. Here, we investigated whether the impairment of senescence preventing mechanisms can be efficiently counteracted by exposure of human adipose-derived stem cells to radio electric asymmetrically conveyed fields by an innovative technology, named Radio Electric Asymmetric Conveyer (REAC). Due to REAC exposure, the number of stem cells positively stained for senescence associated ß-galactosidase was significantly reduced along multiple culturing passages. After a 90-day culture, REAC-treated cells exhibited significantly higher transcription of Bmi1 and enhanced expression of other stem cell pluripotency genes and related proteins, compared to unexposed cells. Transcription of the catalytic telomerase subunit (TERT) was also increased in REAC-treated cells at all passages. Moreover, while telomere shortening occurred at early passages in both REAC-treated and untreated cells, a significant rescue of telomere length could be observed at late passages only in REAC-exposed cells. Thus, REAC-asymmetrically conveyed radio electric fields acted on a gene and protein expression program of both telomerase-independent and telomerase-dependent patterning to optimize stem cell ability to cope with senescence progression.

Item Type:Article
ID Code:10165
Status:Published
Refereed:Yes
Uncontrolled Keywords:Stem cell senescence, Radio Electric Asymmetric Conveyer (REAC) exposure, catalytic telomerase subunit (TERT)
Subjects:Area 05 - Scienze biologiche > BIO/10 Biochimica
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze Biomediche
Publisher:Nature Publishing Group
eISSN:2045-2322
Deposited On:09 Oct 2014 09:36

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