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Homologous prime boosting based on intranasal delivery of non-pathogenic invasive Escherichia coli expressing MPT64, decreases Mycobacterium tuberculosis dissemination

Sali, Michela and Dainese, Elisa and Morandi, Matteo and Zumbo, Antonella and Rocca , Stefano and Goussard, Sylvie and Palù, Giorgio and Grillot-Courvalin, Catherine and Delogu, Giovanni and Manganelli, Riccardo (2014) Homologous prime boosting based on intranasal delivery of non-pathogenic invasive Escherichia coli expressing MPT64, decreases Mycobacterium tuberculosis dissemination. Vaccine, Vol. 32 (32), p. 4051-4058. ISSN 0264-410X. Article.

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DOI: 10.1016/j.vaccine.2014.05.060

Abstract

Protein-subunit vaccines as boosting strategies against tuberculosis (TB) infection are currently in the pipeline of TB vaccine research. Their main limitation is represented by their poor immunogenicity, which makes it necessary to couple protein-subunits with adjuvant molecules. In this study, we employed replication-deficient invasive Escherichia coli strains to deliver Mycobacterium tuberculosis proteins to the cytoplasm of non-phagocytic eukaryotic cells using various priming and prime-boosting vaccination protocols. Our results demonstrate that intranasal administration of invasive E. coli expressing the M. tuberculosis protective antigen MPT64 to mice primed with a recombinant BCG strain over-expressing MPT64 on its surface, decrease bacterial burden in mice spleens. Our data suggest that replication-deficient invasive E. coli may represent a suitable platform for BCG/rBCG priming followed by homologous-boosting immunization strategies.

Item Type:Article
ID Code:10089
Status:Published
Refereed:Yes
Uncontrolled Keywords:Tuberculosis, attenuated vaccines: Mycobacterium tuberculosis, bactofection
Subjects:Area 07 - Scienze agrarie e veterinarie > VET/03 Patologia generale e anatomia patologica veterinaria
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Medicina Veterinaria
Publisher:Elsevier
ISSN:0264-410X
Deposited On:15 Sep 2014 11:49

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