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In Tobacco BY-2 cells xyloglucan oligosaccharides alter the expression of genes involved in cell wall metabolism, signalling, stress responses, cell division and transcriptional control

González-Pérez, Lien and Perrotta, Lara and Acosta, Alexis and Orellana, Esteban and Spadafora, Natasha D. and Bruno, Leonardo and Bitonti, Maria Beatrice and Albani, Diego Maria and Cabrera, Juan Carlos and Francis, Dennis and Rogers, Hilary J. (2014) In Tobacco BY-2 cells xyloglucan oligosaccharides alter the expression of genes involved in cell wall metabolism, signalling, stress responses, cell division and transcriptional control. Molecular Biology Reports, Vol. 41 (10), p. 6803-6816. ISSN 0301-4851. eISSN 1573-4978. Article.

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DOI: 10.1007/s11033-014-3566-y

Abstract

Xyloglucan oligosaccharides (XGOs) are breakdown products of XGs, the most abundant hemicelluloses of the primary cell walls of non-Poalean species. Treatment of cell cultures or whole plants with XGOs results in accelerated cell elongation and cell division, changes in primary root growth, and a stimulation of defence responses. They may therefore act as signalling molecules regulating plant growth and development. Previous work suggests an interaction with auxins and effects on cell wall loosening, however their mode of action is not fully understood. The effect of an XGO extract from tamarind (Tamarindus indica) on global gene expression was therefore investigated in tobacco BY-2 cells using microarrays. Over 500 genes were differentially regulated with similar numbers and functional classes of genes up- and down-regulated, indicating a complex interaction with the cellular machinery. Up-regulation of a putative XG endotransglycosylase/hydrolase-related (XTH) gene supports the mechanism of XGO action through cell wall loosening. Differential expression of defence-related genes supports a role for XGOs as elicitors. Changes in the expression of genes related to mitotic control and differentiation also support previous work showing that XGOs are mitotic inducers. XGOs also affected expression of several receptor-like kinase genes and transcription factors. Hence, XGOs have significant effects on expression of genes related to cell wall metabolism, signalling, stress responses, cell division and transcriptional control.

Item Type:Article
ID Code:10086
Status:Published
Refereed:Yes
Uncontrolled Keywords:BY-2 cells, cell cycle, cell walls, microarray analysis, nicotiana tabacum, xyloglucan oligosaccharides
Subjects:Area 05 - Scienze biologiche > BIO/04 Fisiologia vegetale
Divisions:001 Università di Sassari > 01-a Nuovi Dipartimenti dal 2012 > Scienze della Natura e del Territorio
Publisher:Springer Netherlands
ISSN:0301-4851
eISSN:1573-4978
Deposited On:15 Sep 2014 11:40

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